The article examines the specifics of manifesting a sense of humor and emotional burnout among the preschool teachers. Particular attention is paid to the fact that manifesting the sense of humor can influence the prevention of professional deformations among teachers. The relevance of the research problem is conditioned: firstly, by insufficient studying the relationship between the sense of humor and the emotional burnout syndrome among preschool teachers; secondly by its ambiguity. Two opposing points of view on the interaction of burnout and professional deformation are presented. A number of researchers’ results reflect a negative relationship between a sense of humor and professional deformation. At the same time there is a number of directly opposed studies showing the positive effect of humor on preventing emotional burnout. The use of correlation analysis (Spearman’s r-test) showed both negative and positive statistically significant relationships between humor styles and attitudes towards humor and phases of burnout (p < 0.05). The obtained results allowed formulating a number of conclusions regarding the fact that the relationship has a differentiated nature consisting of the positive impact of the affiliate style on emotional burnout, as well as negative manifesting of humor associated with aggressive style and the fear of looking funny which are less protective against emotional burnout.